LOCUS       Amp;_beta-lactam        5350 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pAP3neo DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    Amp; beta-lactamase; neomycin phosphotransferase; neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Kobori M, Ikeda Y, Nara H, Kato M, Kumegawa M, Nojima H, Kawashima 
            H.
  TITLE     Large scale isolation of osteoclast-specific genes by an improved 
            method involving the preparation of a subtracted cDNA library
  JOURNAL   Genes Cells 3 (7), 459-475 (1998)
  PUBMED    9753427
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Nojima H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-1997) Hiroshi Nojima, Research Institute for 
            Microbial Diseases, Osaka University, Department of Molecular 
            Genetics; Yamadaoka 3-1, Suita, Osaka 565-0871, Japan 
            (E-mail:snj-0212@biken.osaka-u.ac.jp, Tel:81-6-6875-3980, 
            Fax:81-6-6875-5192)
REFERENCE   3  (bases 1 to 5350)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5350)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells"; date: "1998"; volume: "3"; issue: "7"; pages: "459-475"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAY-1997) Hiroshi Nojima, Research Institute for Microbial 
            Diseases, Osaka University, Department of Molecular Genetics"; 
            volume: " Yamadaoka 3-1, Suita, Osaka 565-0871, Japan 
            (E-mail:snj-0212@biken.osaka-u.ac.jp, Tel:81-6-6875-3980, Fax"; 
            pages: "81-6-6875-5192"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    43..177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      47..268
                     /label=SV40 late polyadenylation signal
                     /note="SV40 late polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    428..568
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(754..1342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1516..2373)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2374..2478)
                     /label=AmpR promoter
     rep_origin      complement(2504..2959)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3101..3117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3169..3466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3596..4387
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    complement(4442..4576)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4654..4983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          5022..5118
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        5188..5206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5303..5321)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccttct gaggcggaaa gaaccagccg gatccctcga gggatccaga catgataaga
       61 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      121 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
      181 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
      241 agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt
      301 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      361 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      421 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
      481 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      541 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
      601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1561 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1621 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1681 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1741 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1801 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1861 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1921 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1981 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2041 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2101 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2161 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2221 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2281 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2341 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2401 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2461 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     2521 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2581 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     2641 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2701 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2761 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2821 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2881 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2941 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3001 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     3061 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     3121 cgcgtcgacg gtatcgataa gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3301 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3361 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3421 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3481 gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
     3541 gtcttttatt tcaggtcccg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     3601 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3661 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3721 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     3781 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3841 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3901 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3961 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4021 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4081 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4141 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4201 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4261 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4321 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4381 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgccg
     4441 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4501 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4561 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4621 ggtgtgggag gttttttagc ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4981 aaaaagctcc tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt
     5041 cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga
     5101 tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgctgcag
     5161 gagctcggac cgggccctta ggacgcgtaa tacgactcac tatagggaat tcgacgtcta
     5221 gatcttaagg cgcgccaagg ggttggccac gtggtaacca cggggtggct agctagggat
     5281 aacagggtaa tatagcggcc gccctttagt gagggttaat ttaaatcgta cgtcgcgatt
     5341 aattaaccgc
//
